BIO 298 Special Topics: Biotechnology Instrumentation II
Prerequisites: CHM 130, BIO
156 and BIO 205 or CHM 151 and BIO 181 or permission of instructor.
Course
Description: 4 credit hours (3 lecture;
3 lab) Principles and methodologies of
protein expression isolation, identification and purification.
Performance
Objectives
- upon completion of the course, the student will be able to do the following:
1.
Implement the scientific method to answer specific questions
2.
Calibrate and use common and basic types of laboratory
equipment.
3.
Read and follow and instruction manual for lab equipment and
lab procedures.
4.
Calculate and prepare solutions in various concentrations.
5.
Handle store and dispose of commonly used chemicals and
biohazardous materials.
6.
Maintain a lab notebook documenting lab procedures, data
collection, problems solving episodes, graphing and analysis and future
recommendations.
7.
Assess personal compliance with lab safety procedures.
8.
Learn fundamental techniques routinely used in biotechnology
laboratories related to protein biochemistry, cell culture and immunology..
Course Outline
I.
Fundamentals of protein chemistry.
II.
Protein purification and identification
A.
chromatographic methods
B.
polyacrylamide gel electrophoresis
C.
western blotting
D.
enzyme linked immunosorbent assay (ELISA).
III.
Fundamentals of immunology and serology.
IV.
Cell and tissue culture
A.
bacterial cultures
B.
eukaryotic cultures
C.
cell counting